2018-02-12T17:27:33.000Z2025-05-15 16:41:16
description
similar to protease
tRNA uridine hydroxylase (in complex with [[protein|YrrO]])
locus
BSU27350
BSU_27350
geneLength
927
930
function
unknown
introduction of 5-methoxyuridine modification in tRNA (U34)
product
unknown
tRNA uridine hydroxylase
ec
names
yrrN
yrrN
trhP1
outlinks
bsu
BSU27350
BSU_27350
Gene
Coordinates
2,794,147 → 2,795,076
2,794,147 2,795,076
The protein
Protein family
peptidase U32 family (according to Swiss-Prot)
peptidase U32 family (with [[protein|YrrO]], according to UniProt)
The protein
Paralogous protein(s)
[[protein|YrrO]]
[[this]]
Biological materials
Mutant
MGNA-A008 (yrrN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/8 NBRP B. subtilis, Japan]
BKE27350 (Δ[[gene|yrrN]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27350 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGACGTTCACCTCTTCTT, downstream forward: _UP4_TATTAATCAAAAGGAGGTTA
BKK27350 (Δ[[gene|yrrN]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27350 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGACGTTCACCTCTTCTT, downstream forward: _UP4_TATTAATCAAAAGGAGGTTA
MGNA-A008 (yrrN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/8 NBRP B. subtilis, Japan]
BKE27350 ([[gene|yrrN]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27350 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGACGTTCACCTCTTCTT, downstream forward: _UP4_TATTAATCAAAAGGAGGTTA
BKK27350 ([[gene|yrrN]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27350 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGACGTTCACCTCTTCTT, downstream forward: _UP4_TATTAATCAAAAGGAGGTTA
Gene
Phenotypes of a mutant
50% reduction in mo5U tRNA levels [pubmed|31358606]
The protein
Catalyzed reaction/ biological activity
hydroxylation of U34 in tRNA to give 5-hydroxyuridine (ho5U) (in complex with [[protein|YrrO]]) with prephenate as hydroxyl donor, ho5U is the precursor for 5-methoxyuridine modification (by [[protein|TrmR]]) [pubmed|31358606]
References
Research papers